Single Primer Mediated Cloning of P53 Regulated Genes

Our objective is the isolation and haracterization of previously unidentified gene sequences that mtranscriptional control by the p53 tumor suppressor. Using a single primer-mediated amplification teInverted Repeat Amplification (TIRA), we have successfully isolated three such sequences from humanDNA. The immediate objectives of this proposal are to demonstrate that genes linked to these sequenctranscriptional regulation of p53. Also, additional putative binding sites will be isolated using TIpotential as p53-mediated elements. The isolation and identification of p53-regulated genes will leareagents to be used in characterizing key intermediates involved in cell cycle progression and DNA raberrant regulation of these genes may be significant in many forms of cancer, including breast, ovalong-term goals of this research are the in vitro expression of these p53-regulated genes, and the dantibodies against the polypeptide. These antibodies will prove to be extremely useful in designingdiagnostic and -prognostic indicators for the detection of these cancers.