Method for Control of Biting Midges Using Odor-Baited Traps

We propose to develop a method of trapping biting midges including Culicoides. Biting midges are economically important insect pests. Their presence in large numbers can have a detrimental impact on land development and tourism. In addition, these insects are the vector of bluetongue, an arbovirus which is transmitted in cattle and sheep during repeated blood-feeding by biting flies. Our approach is to identify attractants used by Culicoides locate potential vertebrate hosts. In Phase I of this grant, we identified and characterized the carbon dioxide receptor neuron found in sensilla on the maxillary palps of this insect. However, these maxillary palps sensilla were not sensitive to any other chemical tested. Therefore, in Phase II, we will continue these electrophysiological studies on the antenna. We expect to locate neurons sensitive to other chemical attractants. Concurrent with these studies, we propose a series of field test aimed at determining what signals Culicoides use to orient to host. Such studies will lead to the development of an effective trap for Culicoides and should be useful for control of this economically- important pest species.