SBIR/STTR Research Award
EXPRESSION OF HUMAN PROTEIN C IN MAMMALIAN CELLS
Award amount
$279,000Program and phase
SBIR · Phase IIAward year
1987Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- Not reported
- Recipient company
- Zymogenetics Inc
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
A FULL-LENGTH CODING SEQUENCE FOR HUMAN PROTEIN C WILL BE CLONED. THIS WILL BE DONE EITHER BY OBTAINING A 5' END CLONE FROM A LIVER CDNA LIBRARY OR BY FUSING A GENOMIC DNA SEQUENCE TO CDNA, WHICH LACKS AN AMINOTERMINAL CODING SEQUENCE. ONCE THIS CLONE IS COMPLETE IS WILL BE INTRODUCEDINTO AN EXPRESSION VECTOR DESIGNED FOR HIGH-LEVEL EXPRESSIONIN MAMMALIAN CELLS. THIS CONSTRUCTION WILL BE TRANSFECTED INTO BHK CELLS AND EXPRESSED PROTEIN C ACTIVITY MEASURED. PROTEIN C WILL BE ASSAYED BY ELISA AND FOR ANTICOAGULANT ACTIVITY.
Technology keywords
Not reported