SBIR/STTR Research Award
THIS PROJECT WILL PRODUCE MOUSE AND RAT MONOCLONAL ANTIBODIES TO THE LEUKEMOGENIC HUMAN RETROVIRUS, HTLV-I.
Award amount
$50,000Program and phase
SBIR · Phase IAward year
1986Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- Not reported
- Recipient company
- NEUGENESIS CORPORATION
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
THIS PROJECT WILL PRODUCE MOUSE AND RAT MONOCLONAL ANTIBODIES TO THE LEUKEMOGENIC HUMAN RETROVIRUS, HTLV-I. STABLE, IMMUNOGLOBULIN-SECRETING HTLV-I ELISA-REACTIVE SUBCLONES WILL BE ISOLATED AND THEIR VIRAL ANTIGEN SPECIFICITY CONFIRMED BY IMMUNOPRECIPITATION, WESTERN BLOTTING, AND IMMUNOFLUORESCENCE ANALYSIS. THE BINDING CONSTANTS OF THE ANTIBODIES PRODUCED WILL BE DETERMINED. ITIS ANTICIPATED THAT IN A PHASE II PROJECT, FINE SPECIFICITY MAPPING OF THE ANTIBODIES WILL BE PERFORMED AND THEIR BIOLOGICAL EFFECTS CHARACTERIZED. PRELIMINARY STUDIES, RESULTING IN THE SUCCESSFUL GENERATION OF A MURINE MONOCLONAL ANTIBODY TO AN HTLV-I ANTIGEN, HAVE ALREADY DEMONSTRATED THE FEASIBILITY OF THE PROPOSED APPROACH. SUCCESSFUL GENERATION OF HIGH AVIDITY MOUSE AND RAT MONOCLONALS SHOULD PROVIDE REAGENTS WITH IMPROVED DIAGNOSTICPOTENTIAL AND POSSIBLE THERAPEUTIC UTILITY.
Technology keywords
Not reported