Biocatalytic Polymer Skin Adhesives

This Phase II STTR proposal describes the development of a prototype enzyme-containing adhesive capable of attaching biocatalytic activity directly to skin. The adhesive, when containing organophosphorus-hydrolyzing enzymes, shall serve to protectunderlying skin from otherwise toxic applications of nerve agents and pesticides. Agentase, a recognized world leader in developing polymer-based carriers for enzyme catalysis, in collaboration with the Midwest Research Institute, shall demonstrate thefeasibility of providing personal protection from hazardous chemicals to individuals via enzyme-based skin adhesives.Having successfully demonstrated the viability of synthesizing biocatalytic adhesives with significant enzyme activity and excellent physical properties during Phase I, Phase II activity is directed at developing a prototype adhesive for protection againstOPs. More specifically, proposed work includes optimization of the processes for applying adhesives in a reproducible and uniform fashion, enhancing their capacity for decontamination, and ensuring polymer as well as polymerization compatibility with skintissues. Phase II research shall also provide a clear demonstration that enzyme-containing skin adhesives can be effectively used to protect a small animal model from the debilitating effects of OP exposures. Successful completion of the aims describedin this Phase II STTR proposal will provide a novel biopolymer skin adhesive for protection against organophosphorus contamination from nerve agents and pesticides. The addition of active biomolecules, such as enzymes, to a skin-compatible biopolymeradhesive has great potential in facilitated wound healing.