SBIR/STTR Research Award
THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC A-D-MANNOSIDASE FROM HUMAN PLACENTAS AND BOVINE KIDNEYS.
Award amount
$50,000Program and phase
SBIR · Phase IAward year
1986Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- Not reported
- Recipient company
- Genzyme Corp.
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC A-D-MANNOSIDASE FROM HUMAN PLACENTAS AND BOVINE KIDNEYS. IT IS ANTICIPATED THAT BY EMPLOYING A COMBINATION OF CHROMATOGRAPHIC TECHNIQUES ON AFFINITY, HIGH-PERFORMANCE ANION EXCHANGE, AND/OR HIGH PERFORMANCE GEL FILTRATION MATRICES, HOMOGENEOUS ACIDIC A-D-MANNOSIDASE WILLBE OBTAINED. THIS WILL PERMIT THE GENERATION OF POLYCLONAL AND MONOCLONAL ANTIBODIES DURING PHASE II. FURTHERMORE, THEPURIFIED ENZYME AND ANTISERUM WILL PROVIDE A MEANS FOR CLONING THE GENE FOR ACIDIC A-D-MANNOSIDASE. THE ABSENCE OFTHIS ENZYME IN THE LYSOSOMAL STORAGE DISORDER, MANNOSIDOSIS,WHICH OCCURS IN BOTH MAN AND CATTLE, MAKES IT AN IDEAL CANDIDATE FOR STUDYING THE FEASIBILITY OF GENE REPLACEMENT THERAPY.
Technology keywords
Not reported