AN ADVANCED ELECTROCHEMICAL SENSOR FOR THE SIMULTANEOUS ANALYTICAL MEASUREMENT OF THREE CHEMICAL SPECIES

THE REQUIREMENTS OF COMPLEX SAMPLE MONITORING PRESENT A GROWING NEED FOR A NEW GENERATION OF HIGHLY SPECIFIC ENZYME ELETRODES WITH MULTI-ANALYTE, MULTI-SIGNAL CAPABILITY. THIS PROJECT'S OBJECTIVE IS TO ESTABLISH THE FEASIBILITY OF NOVEL ELECTROCHEMICAL SENSORS BASED ON THE COMBINATION OF IMMOBILIZED ENZYMES AND COLLOIDAL GOLD TECHNOLOGIES. THE SPECIFIC GOAL TO BE PURSUED IN PHASE I IS TO DEMONSTRATE THE FEASIBILITY OF AN IN-VITRO LIPID PROFILE DIAGNOSTIC DEVICE FOR THE SIMULTANEOUS DETECTION OF TOTAL CHOLESTEROL, HIGH DENSITY LIPOPROTEINS, AND TRIGLYCERIDES USING A DISPOSABLE BIOSENSOR. THIS LIPID PROFILE TESTING IS AN IMPORTANT PART OF CURRENT RECOMMENDATIONS FOR REDUCING CARDIAC RISK AND FOR MONITORING THERAPY FOR MILLIONS OF INDIVIDUALS WITH CARDIOVASCULAR DISEASE. DEPOSITION OF COLLOIDAL GOLD WITH IMMOBILIZED ENZYMES ONTO SEPARATE CHANNELS OF A MULTI-CHANNEL SURFACE TO PROVIDE SPATIALLY RESOLVED, MULTI-SIGNAL ENZYME ELECTRODES CAN ULTIMATELY LEAD TO A NEW GENERATION OF MULTI-ANALYTE SENSORS.