AN EFFICIENT METHOD FOR GENERATING FULL-LENGTH CDNA

GOLD BIOTECHNOLOGY, INC. IS STUDYING A NEW METHOD FOR GENERATING FULL-LENGTH CDNA. THIS METHOD SHOULD BE SIMPLE AND EFFICIENT AND THUS ALLOW CLONING OF BOTH RARE AND ABUNDANT MRNA. THIS METHOD IMPROVES UPON OTHER PROCEDURES IN THE FOLLOWING WAYS: (1) NO S1 NUCLEASE IS REQUIRED FOR CLONING CDNA INTO THE VECTOR, AND (2) TAILING OF CDNA IS UNNECESSARY FOR BOTH INSERTION OF THE CDNA INTO THE VECTOR AND CIRCULARIZATION OF CDNA VECTOR HYBRID. THE SYNTHESIS OF FULL-LENGTH CDNA CAN BE EXPLOITED TO PRODUCE IMPORTANT COMMERCIAL PRODUCTS. THESE INCLUDE MATERIALS SUCH AS GROWTH FACTORS, HORMONES, AND IMMUNOREGULATORY MOLECULES. IN ADDITION TO DIRECT COMMERCIAL PRODUCTS, THERAPEUTIC USE OF FULL-LENGTH CDNAS MAY BECOME IMPORTANT AS ANTISENSE MRNA TECHNOLOGIES ARE IMPROVED. THIS PROJECT WILL DEMONSTRATE THE SUPERIORITY OF THE PROPOSED CDNA METHOD USING GLOBIN MRNA, A HOMOGENEOUS TEMPLATE.