Polymer Stationary Phases for Hplc

We will develop procedures for in situ preparation of polymer packed columns for high performance lchromatography (HPLC). The program is based on the observation that poly(vinylbenzyl chloride) formuporogenic solvents and other additives polymerize to form uniform connected spheres a few micrometerplan to prepare the polymer in situ in an HPLC column. The procedure would involve pouring a degassereactants solution of reactants into the column and heating to 85 degrees C to cause free radical poplace. It will be much simpler and faster to apply than conventional column packing procedures. Furtuniformity and small size of the sphere should lead to improved chromatographic efficiencies comparepacked by conventional technology. The resulting product will be polymer packed columns that provideefficiencies at modest cost. Phase I will involve preparation and evaluation of various phases. Feasif the polymer phases prepared in situ demonstrate chromatographic efficiencies comparable to commer