THE AIM OF THIS PROJECT IS TO SYNTHESIZE NOVEL ACRIDINIUM ESTERS FOR USE IN CHEMILUMINESCEENCE IMMUNOASSAYS.

THE AIM OF THIS PROJECT IS TO SYNTHESIZE NOVEL ACRIDINIUM ESTERS FOR USE IN CHEMILUMINESCEENCE IMMUNOASSAYS. ACRIDINIUM ESTERS HAVE A GREAT POTENTIAL FOR USE IN THE EXPANDING NON-ISOTOPIC IMMUNODIANOSTICS MARKET. THE CHEMISTRY ASSOCIATED WITH THEIR USE AS LABELS FOR IMMUNOASSAYS IS NOT WELL UNDERSTOOD. THE CHEMISTRY OF THIS CLASS OF COMPOUNDS WILL BE EXAMINED TO PROVIDE IMPROVED, HIGH EFFICIENCY, MORE STABLE LABELS. VARIOUS CHAIN LENGTH MODDIFICATIONS WILL BE MADE TO GIVE ESTERS WHICH WILL HAVE VARYING SPACE BETWEEN PROTEIN AND LABEL. DIFFERENT COUPLING GROUPS WILL BE USED, SO THAT PROTEINS CAN BE LABELED ON LYSINE, CYSTEEINE OR TYROSINE, WITH IMPROVED SOLUBILITY OF THE CONJUGATES. VARIOUS SUBSTITUTIONS ON THE PHENYL ESTER RING WILL BE MADE TO IMPROVE THE STABILITY OF THE ACRIDINIUM ESTERS IN AQUEOUS SOLUTIONS. DERIVATIVES OF THE EMITTER FOR THE CHEMILUMINESCENT REACTIONI, NMA, WILL BE SYNTHESIZEED AND EXPLORE THE CHEMISTRY OF ENERGY TRANSFER DONORS. THIS PROJECT WILL PROVIDE BETTER UNDERSTANDING OF ACRIDINIUM ESTER CHEMISTRY AND THEIR EVENTUAL COMMERCIALIZATION AS LABELS IN IMMUNOASSAYS.