SBIR/STTR Research Award
AFFINITY PURIFICATION OF PARAINFLUENZA VIRUS GLYCOPROTEINS
Award amount
$498,100Program and phase
SBIR · Phase IIAward year
1986Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- Not reported
- Recipient company
- MOLECULAR ENGINEERING CORP.
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
IT IS PROPOSED TO PURIFY THE TWO ENVELOPE GLYCOPROTEINS, HN AND F, FROM HUMAN PARAINFLUENZA TYPE 3 (PI3) VIRUS BY AFFINITY CHROMATOGRAPHY USING SPECIFIC MONOCLONAL ANTIBODIES AND TO INCORPORATE THEM INTO LIPID VESICLES. THE EXPERIMENTAL APPROACH INVOLVES IMMOBILIZATION OF SPECIFIC MONOCLONAL ANTIBODY ON A SEPHAROSE 4B MATRIX. PURIFIED VIRUS OR INFECTED CELLS WILL BE USED AS THE SOURCE OF ANTIGENS FOR ADSORPTION AND PURIFICATION OF SPECIFIC GLYCOPROTEINS THROUGH AFFINITY COLUMNS. THE TWO PURIFIED ANTIGENS WILL BE INCORPORATED IN DEFINED PROPORTIONS INTO LIPID VESICLES. THIS RESEARCH WILL FACILITATE THE DEVELOPMENT OF MORE EFFECTIVE VACCINES AND WILL LEAD TO A PHASE II PROPOSAL IN WHICH THE EFFICACY OF THE RECONSTITUTED GLYCOPROTEIN-CONTAINING VESICLES WILL BE EVALUATED AS A VACCINE IN EXPERIMENTAL ANIMALS.
Technology keywords
Not reported