LOCI FOR USE IN ROUTINE SCREENING IN THE L5178Y MOUSE LYMPHOOUR AIM IS TO DETERMINE WHETHER MUTATION AT THE TK LOCUS IN L5178Y MOUSE LYMPHOMA CELLS IS REPRESENTATIVE OF MUTATION THROUGHOUT THE ENTIRE MAMMALIAN GENOME.

LOCI FOR USE IN ROUTINE SCREENING IN THE L5178Y MOUSE LYMPHOOUR AIM IS TO DETERMINE WHETHER MUTATION AT THE TK LOCUS IN L5178Y MOUSE LYMPHOMA CELLS IS REPRESENTATIVE OF MUTATION THROUGHOUT THE ENTIRE MAMMALIAN GENOME. MUTATION INDUCTION WILL BE COMPARED AT THREE LOCI IN L5178Y CELLS AND TWO LOCI IN CHINESE HAMSTER OVARY (CHO) CELLS. CHEMICALS WITH DIFFERENT MODES OF ACTION IN THE CELL WILL BE EXAMINED. IT IS EXPECTED THAT EACH LOCUS WILL HAVE A DIFFERENT RESPONSE PATTERN TO DIFFERENT CHEMICAL CLASSES. THE GOAL OF THE STUDY IS TO PROVIDE EVIDENCE FOR THE USE OF THE L5178Y TK/ MUTAGENESIS ASSAY IN THE DETECTION OF POTENTIAL CARCINOGENS. THE RESULTS OBTAINED IN PHASE I WITH MODEL COMPOUNDS WILL PROVIDE THE BASIS FOR AN EXTENSIVE STUDY IN PHASE II OF THE RESPONSE OF THE LOCI TO MUTAGENS/CARCINOGENS. THE PROJECT WILL ALSO COMPARE RESULTS OF THE L5178Y MUTAGENESIS ASSAY WITH RODENT CARCINOGENICITY RESULTS TO DETERMINE WHETHER A POSITIVE CORRELATION EXISTS BETWEEN THEM. A POTENTIAL COMMERCIAL APPLICATION OF THE PROJECT IS THE VALIDATION OF MULTIPLE LOCI IN L5178Y CELLS FOR ROUTINE MUTAGENICITY TESTING.