USE OF LIQUID CARRIER'S IN TISSUE CULTURE FOR TISSUE CULTUREFOR AERATIC

THE LIMITATIONS OF EXISTING EQUIPMENT AND PROCESS TECHNOLOGY FOR THE PRODUCTION OF PRODUCTS DERIVED FROM TISSUE CULTURE IS WELL RECOGNIZED IN THE INDUSTRY. THIS RESEARCH PROJECT IS FOCUSED ON THE PROBLEM OF AERATION IN THE CELL CULTIVATION PROCESS OF GROWING TISSUE CULTURES. THIS PROJECT INTRODUCES A NEW TECHNOLOGY OF USING CERTAIN LIQUID PERFLUORINATED CHEMICALS (PFC) WHICH ARE IMMISCIBLE IN AQUEOUS MIXTURES, BIOLOGICALLY INERT AND HAVE AFFINITIES FOR 02 AND C02 OFTEN REACHING 5-10 TIMES THOSE OFWATER AS A TRANSFER MEDIA. THIS PROCESS WILL USE CELL LINESTHAT GROW IN SUSPENSION CULTURE MEDIA DEVOID OF SERUM. THE DISPERSION OF PFC IN THE AQUEOUS CULTURE MEDIUM WILL FACILITATE THE RECOVERY AND RECYCLING OF PURE PFC. THE PFC WILL BE CIRCULATED THROUGH A DRAFT TUBE REACTOR TO CREATE BULK MIXING. GAS EXCHANGE OCCURS DUE TO THE DISEQUILIBRIUM BETWEEN THE TWO PHASES. WE WILL ATTEMPT TO QUANTIFY THE OXYGEN TRANSPORT RATES REQUIRED TO SUPPORT HIGHCULTURE DENSITIES. THIS RESEARCH COULD REMOVE ONE OF THE TECHNICAL OBSTACLES INGROWING TISSUE CULTURES IN HIGH CELL DENSITIES WHILE LOWERING THE COST OF CELL CULTIVATION. CONSIDERATION WILL BE GIVEN TO USE OF THIS NEW PROCESS IN MICROGRAVITY CONDITIONS TO DETERMINE IF THE UNIQUE ATTRIBUTES OF SPACE USING THIS NEW TECHNOLOGY WILL ALLOW INTER-ATOMIC AND INTER-MOLECULAR FORCES TO ENHANCE THE PROCESS.