Real Time Imaging System for Tritiated Biological Sample

Radioactive labeling of proteins using tritium is a very important technique used in neurological ridentify, isolate, and investigate the expression of proteins in biological systems. It is often necradiolabel for this work because 3H in vivo labeled proteins retain their conformal structure and arassays. Currently, many 3H-labeled binding assays are done using inconvenient, time consuming, and etechniques involving scintillation cocktail measurement because no reliable and sensitive tritium dequantify the radioactivity directly and non-destructively from the sample. We will develop a novel sdetector system capable of measuring the spatial activity distribution of tritiated biological specilabor intensive liquid scintillation fluid techniques. This system is based on a large area array ofphotodiodes (APDs) which will be capable of directly sensing the low energy radiation emissions of tsamples collected on filter paper. A system based on the parallel acquisition of many such pixil elesuperior sample throughput for a wide variety of 3H-binding assays and thus will represent a signifiincrease for many procedures while greatly reducing the quantity of chemical and radioactive waste t