Device for the Uncontaminated Collection of Multiple Microbial Samples

Not Available The objective of this research project is the development of an Autonomous Microbial Sampler (AMS) that will obtain uncontaminated and exogenous DNA-free microbial samples from deep-sea hydrothermal vents and other aquatic ecosystems. Sampling with the AMS may be conducted using manned submersibles, Remotely Operated Vehicles (ROV), Autonomous Underwater Vehicles (AUV) or during hydrocast operations on ships. The modular device consists of a titanium sample nozzle for sampling potentially hot samples (-250 C) and fluid handling components housed within a stainless steel frame for collection of six independent filtered or unfiltered samples. An on board microcomputer permits sampling to be controlled by the investigator, external devices (e.g. AUV computer) or internal programming. Temperature, volume pumped and other parameters are recorded during sampling. Detailed engineering design of the device and choice of construction of a prototype AMS, and a program of thorough laboratory and field testing of it¿s operation. Minor design changes based on testing results will be incorporated into the device. Field testing will be conducted during an upcoming R/V Atlantis/DSV Alvin cruise to a deep water hydrothermal vent site off the U.S. or Mexican West coast in spring 2000. Test hydrocast water column sampling will be conducted during an R/V Endeavor cruise to the Sargasso Sea in spring 2001.