Mapping Sequences on DNA with Fluorescent Probes

Funds are requested for development of a method for obtaining a physical map of any group of specifsequences on a sample DNA molecule. The linear positions of the sequences are obtained by observatiomicroscope, of oligonucleotide probes tagged with a fluorescent markers. In the initial developmentlong DNA from the bacterial virus lambda is proposed as a model sample DNA, since the complete sequethis DNA. When applied to genomic DNA obtained from human cells, the method has the potential to obtsequences in the vicinity of any unique sequence in the genome without purification or cloning stepscurrent technology. It is anticipated that the mapping method would initially be used in the Human Gdetermine the relative positions of clones of small segments of the genome; thus, speed the assemblythese segments into the genomic sequence. However, the same mapping method might later be developedDNA probe assay with the ability to simultaneously detect multiple genetic defects.