APPLIED EMBRYOLOGY TECHNIQUES IN NONHUMAN PRIMATES

THIS PROJECT IS THE FIRST PHASE OF A LONG-TERM, JOINT VENTURE BETWEEN HELIX INTERNATIONAL CORPORATION AND THE UNIVERSITY OF SOUTHWESTERN LOUISIANA TO ESTABLISH A COLONY OF MONOZYGOTIC NONHUMAN PRIMATE TWINS FOR BIOMEDICAL RESEARCH MODELS AND TO DEVELOP THE TECHNOLOGY AND IN-HOUSE EXPERTISE FOR INITIATING WORK IN INTERSPECIES EMBRYO TRANSFER IN ENDANGERED PRIMATES. DURING THE COURSE OF THIS RESEARCH, A NONSURGICAL EMBRYO-RECOVERY TECHNIQUE WILL BE PERFECTED FOR RECOVERY OF UTERINE-STAGE EMBRYOS FROM RHESUS MONKEY 7MACACA MULATTA) FEMALES. RECOVERED EMBRYOS (MORULA TO BLASTOCYST STAGES) WILL BE MICROSURGICALLY BISECTED INTO MONOZYGOTIC TWIN DEMI-EMBRYOS (DE). THE DE WILL BE USED TO COMPARE TWO CULTURE SYSTEMS (CMRL MEDIUM ALONE OR WITH COCULTURED ENDOMETRIAL FIBROBLASTS) FOR MAINTAINING IN VITRO VIABILITY OVER 24 HOURS. FOLLOWING CULTURE, THE DE WILL BE STAINED WITH FLUORESCEIN DIACETATE TO ASSESS VIABILITY. THE DE WILLTHEN BE FIXED AND STAINED TO COUNT THE NUMBER OF CELLS OR FROZEN FOR SUBSEQUENT ASSESSMENT OF VIABILITY FOLLOWING CRYOPRESERVATION. ULTIMATE SUCCESS IN PRODUCING A SUBSTANTIAL COLONY OF MONOZYGOTIC NONHUMAN PRIMATE TWINS WILL PROVIDE UNIQUELY VALUABLE ANIMAL MODELS FOR BIOMEDICAL RESEARCH.