A Rapid Point of Care Test for the Detection of Antibodies to Measles and Rubella

Instadiagnostics overall goal is to bring a majority of laboratory tests to the point of care (POC) on our portable platform and provide lab quality results within 15-20 minutes. It consists of an all-inclusive microfluidic cartridge that accepts unprocessed samples (blood) and an electronic reader. Once the sample is added, the cartridge is inserted into the reader and the test is performed without any additional user input. Project Goal: To develop POC measles and rubella IgM and IgG antibody assays to address surveillance and outbreak management needs. Future expansions could include mumps and varicella assays on the same panel. Phase I Objectives: 1: Develop rapid assays for detecting measles and rubella IgM antibodies Immunoassays for measles IgM antibodies and rubella IgM antibodies will be developed and optimized for our cartridge/reader system to maximize the difference in signal between positive and negative plasma samples, ensure reproducibility, and minimize assay time. 2: Demonstrate sensitivity and specificity of at least 80% for each assay with a panel of archived specimens. A CDC provided panel of archived specimens will be tested using the assays developed in Objective 1, with the goal of achieving at least 80% sensitivity and specificity.