SBIR/STTR Research Award

A METHOD FOR INCREASING THE HYBRIDIZATION EFFICIENCY OF DNA AND RNA PROBES

Award identifier d6584d203d0fd500448998b3b2ac81ede6543dce6631077af2d22b6b1ca5108bAwarding agency National Science Foundation

Award amount

$187,000

Program and phase

SBIR · Phase II

Award year

1987

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Award details

Awarding agency
National Science Foundation
Branch
Not reported
Recipient company
Pacific Biotechnology Associat
Proposal award date
Not reported
Contract end date
Not reported
UEI
Not reported
Solicitation number
Not reported
Topic code
Not reported

Research abstract

A METHOD FOR INCREASING THE SENSITIVITY OF NUCLEIC ACID HYBRIDIZATION REACTIONS INVOLVING NON-RADIOACTIVE DNA PROBES IS EXAMINED. PACIFIC BIOTECHNOLOGY RESEARCH ASSOCIATES HAS A PROPRIETARY METHOD WHICH INCREASES THE HYBRIDIZATION EFFICIENCY OF RADIOACTIVELY-LABELED GENETIC PROBES BY OVER 1000%. THIS METHOD WILL BE APPLIED TO COMMERCIALLY-AVAILABLE SYSTEMS WHICH PRODUCE NON-RADIOACTIVE DNA PROBES TO DETERMINE WHETHER THE SAME DEGREE OF AMPLIFICATION CAN BE OBTAINED IN A NON-RADIOISOTOPIC SYSTEM. SEVERAL MODIFICATIONS OF THE BASIC TECHNIQUE WILL BE TESTED IN ORDER TO DETERMINE THE OPTIMAL CONDITION FOR INCREASING THE SIGNAL OBTAINED WITH NON-RADIOACTIVE DNA PROBES.

Technology keywords

Not reported

Agency intelligence

National Science Foundation

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