SBIR/STTR Research Award
IMPROVED ASSAYS FOR SPECIFICALLY MEASURING COLLAGENASE-3
Award amount
$172,306Program and phase
SBIR · Phase IAward year
2001Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- National Institutes of Health
- Recipient company
- ORDER STATUS VEHICLE, INC. (OSV)
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
DESCRIPTION (provided by applicant): Collagenase-3 is a matrix metalloprotease
that is associated with diseases such as cancer and osteoarthritis. Collagenase
3 degrades type II collagen very efficiently in the joint and inhibitors of
this enzyme have been effective at preventing cartilage degradation in ex vivo
and in vivo models of osteoarthritis. Recently we discovered novel substrates
for collagenase 3 that are highly selective for this enzyme using substrate
mapping on protein expression libraries in phage lambda (phage display). One
goal is to use this knowledge to develop a "assay" kit that can measure
collagenase 3 activity directly in biological fluids. We will develop this kit
to include two techniques: fluorescence substrates that are selective for
collagenase 3 and neo-epitope antibodies to the cleavage products of
collagenase 3. This "assay" kit can then be used to follow disease progression
in osteoarthritis, and potentially follow cancer progression, by assessing
collagenase 3 activity as a biological marker.
PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE
Technology keywords
Not reported