HYDROGEN PEROXIDE MONITOR

WE HAVE DEVELOPED HIGHLY SPECIFIC, SENSITIVE DEVICES FOR THEMONITORING OF GLUCOSE, PRIMARY ALCOHOLS, KETONES AND OTHER PRODUCTS. OUR TECHNOLOGY USES A MEDIATOR TO TRANSFER ELECTRONS DERIVED FROM THE ENZYMIC OXIDATION OF THE SUBSTANCE BEING MEASURED, TO AN ELECTRODE HELD AT CONSTANT POTENTIAL. NUMEROUS RESEARCHERS HAVE INVESTIGATED THE TRANSFER OF ELECTRONS FROM AN OXIDASE ENZYME TO AN ELECTRODE. HOWEVER, NONE HAVE ACHIEVED THE CURRENT DENSITIES REQUIRED TO MAKE A REALISTIC DEVICE. WE HAVE ALREADY ACHIEVED (A) HIGH DENSITY ELECTRON TRANSFER BETWEEN A RANGE OF ENZYMES AND ELECTRODES, AND (B) INCORPORATION OF ENZYMES ONTO ELECTRODES TO MAKE REALISTIC MONITORING DEVICES. THE HYDROGEN PEROXIDE MONITOR WILL USE CHEAP CARBON ELECTRODE, HORSE HEART CYTOCHROME C (A COMMERCIALLY AVAILABLE ENZYME) AS A MEDIATOR OF ELECTRON TRANSPORT, AND CYTOCHROME C PEROXIDASE AS THE ACTIVE ENZYMIC AGENT. ELECTRONS FROM THE OXIDATION OF HYDROGEN PEROXIDE AT THE CYTOCHROME C PEROXIDASE WILL BE PASSED VIA THE MEDIATOR, TO THE CARBON ELECTRODE. THE RESULTING CURRENT IN THE EXTERNALCIRCUIT CAN BE USED TO MEASURE THE CONCENTRATION OF HYDROGENPEROXIDE.