A NEW RAPID TEST FOR SALMONELLA DETECTION USING LIPOSOME -ENTRAPPED MOLECULES

THE TESTING FOR SALMONELLA CURRENTLY INVOLVES METHODOLOGY THAT REQUIRES 3-8 DAYS TO COMPLETE AND A FAIRLY SOPHISTICAT ED SKILL LEVEL TO PERFORM. THIS PROJECT IS DESIGNED TO D DEVELOP BOTH MANUAL AND BIOSENSOR-BASED HOMOGENEOUS ASSAYS THAT WILL REDUCE THE DETECTION TIME OF SALMONELLA TO WITHIN 24 HOURS AND BE TECHNICALLY SIMPLER TO PERFORM. THE NOVEL ASPECT IS THE USE OF A LIMPSOME-ENCAPSULATED ENZYME. THE ENZYME WILL BE RELEASED FROM THE LIPOSOME UPON THE INTER ACTION OF SALMONELLA ORGANISMS WITH A MONOCLONAL ANTIBODY ON THE LIPOSOME SURFACE IN THE PRESENCE OF COMPLEMENT. THE ENZYME WOULD NOW BE FREE TO GENERATE A PRODUCT WHICH WOULD CAUSE A COLOR CHANGE IN THE MANUAL TEST AND A MEASURABLE CONDUCTIVITY CHANGE IN THE BIOSENSOR-BASE TEST.