SBIR/STTR Research Award
DEVELOPMEN OF A NEW DNA SEQUENCING INSTRUMENT
Award amount
$50,000Program and phase
SBIR · Phase IAward year
1989Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- Not reported
- Recipient company
- Sybtrel Biotechnology
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
THE OBJECTIVE OF THIS PROJECT IS TO DEVELOP A DNA SEQUENCING INSTRUMENT UTILIZING THE PYROPHOSPHATE-BASED METHOD OF DNA SEQUENCING. THIS IS A NEW METHOD THAT DOES NOT USE ELECTROPHORESIS, RADIOACTIVITY, OR FLUORESCENCE. THE METHOD WORKS BY MEASURING PYROPHOSPHATE GENERATED BY THE DNA POLYMERIZATION REACTION. THE PYROPHOSPHATE IS MEASURED CONTINUOUSLY BY A DEVICE CONSISTING OF A SERIES OF COLUMNS CONTAINING ENZYMES COVALENTLY ATTACHED TO A SOLIDSUPPORT. PHASE I WILL BE DEVOTED TO SOLVING THE REMAINING BIOCHEMICAL PROBLEMS ASSOCIATED WITH THE METHOD. IF THE METHOD IS DEMONSTRATED TO WORK WELL ENOUGH, PHASE II WILL CONSIST OF CONSTRUCTION OF A PROTOTYPE INSTRUMENT SUITABLE FOR LARGE-SCALE PRODUCTION.
Technology keywords
Not reported