Fish Technology to Localize a Prostate Cancer Gene

Although prostate cancer is the most common malignancy among males in the U.S, little is known abougenes involved in prostate carcinogenesis. Cytogenetic and restriction fragment polymorphism data haof 10q in as many as 30% of prostate cancers. In a collaborative effort with a prostate cancer laborEdward Gelmann (Georgetown Univ.), we have shown that the microsatellite marker D10S187 in 10q25 exhheterozygosity (LOH) in approximately one third of primary prostate tumors. Thus, our long-term goalgene(s) in 10q24-25 involved in prostate cancer. Identification and cloning of a prostate cancer suscontribute to our understanding of prostate carcinogenesis. Our primary aim in Phase I is to assessfluorescence in situ hybridization (FISH) technology to detect and finely map 10q deletion events inThe degree of resolution with FISH will be greater than can be obtained with the limited number of 1markers suitable for LOH analysis. Our secondary aims involve increasing the number of YAC and cosmiinformation in the 10q25 region in which the prostate tumor susceptibility gene resides.