Ploidy Application for Added Value and Stock Protection of Domesticated Pacific White Shrimp

This SBIR Phase I research will establish reliable technology to produce tetraploid broodstock of Pacific white shrimp (L. vannamei) using a new, Automated Polyploid Induction System (APIS). We will determine optimal values for tetraploidy induction based on three parameters: time post-fertilization to start shock, shock intensity, and shock duration. These values will allow us to fully develop and apply APIS during Phase II. Tetraploid broodstock thus produced will be used to produce sterile triploid seedstock during Phase II, when their commercial performance will be assessed. Since triploid seedstock are sterile, this technology will protect our investments in stock improvements. Farmed shrimp production has stagnated since 1990 due primarily to viral diseases. Use of hatchery produced, disease-free and -resistant stocks will reduce reliance on wild-caught stocks, the primary source of shrimp diseases. However, genetic improvement of domesticated shrimp stocks requires substantial financial investments. Technology that protects these investments is essential for attracting capital. Once this technology is available, investments in shrimp genetics will follow. We will position our company for expansion by being at the forefront of polyploidy applications in shrimp culture, and by protecting our investments in our shrimp stocks.