Accelerated Detection of Legionellain Water Samples using Electrochemical Impedance-based Devices

The goal of the proposed project is to develop an electrochemical antibody-based sensor, Legionella-EZ™ leverages the highly specific binding of antibodies with the sensitivity of electrochemical impedance analysis. In Phase I we will: 1) demonstrate the capability to discriminate L. pneumophila from other Legionella species, 2) define the sensitivity, selectivity, precision, and limits of detection. 3) validate a total time of detection of less than 1 hour, 4) demonstrate proof-of-concept of the device against culture methods using environmental samples, and 5) develop and validate a test protocol for the prototype device. During Phase II we will: 1) optimize the device performance, 2) adapt commercial-off-the shelf analysis devices to optimize portability and usability, 3) perform a small-scale production run of sensor strips to test against samples from the CDC’s Environmental Legionella Isolation Techniques Evaluation program. Provide key words (8 maximum) to identify the research or technology. Legionella, electrochemistry, impedance spectroscopy, water samples, antibody, sensor Provide a summary of the potential commercial applications of the research. The proposed research will result in a rapid, cost-effective, and easy to use electrochemical impedance-based device.