Next Generation Root-Pest Resistance Research and Screening Tool

This project involves development of a non-destructive, non-invasive technique for visualization and characterization of root damage by devastating root pests using digital x-ray imaging. To allow use of x-rays, plants are grown in a low density artificial rooting medium that supports healthy plant growth and allows infection by root pests. This project will show the proof of concept that (1) the entire cycle of root pest infection can be carried out in the artificial medium and (2) root damage assessments can be carried out using image analysis tools. In this project two model systems will be used: root knot nematodes on peppers and; western corn rootworm on corn. A root analysis system based on non-invasive imaging will greatly increase the scope of possible experiments on plant root pests and help speed the techniques used for damage assessment.