Automated Sample Collection and Concentration System for Multiple Pathogens in Water

The goal of this research project is to develop a simple, rapid and highly automated sample collection system that will concentrate a broad range of pathogens simultaneously. The system will integrate continuous flow centrifugation with an innovative positively charged filter so that large (i.e. protozoan parasites and bacteria) and small (i.e. enteric viruses) biologically active particles can be concentrated in a single procedure. The system developed in this research project will be fully compatible with current and commonly employed analytic detection techniques and other advanced detection methods such as real-time PCR, DNA microchip arrays, as well as other biosensors. This innovative sample collection and concentration device will provide sample concentrates for the direct detection of waterborne pathogens using molecular techniques or biosensors for routine monitoring water quality as well as an emergency response platform that will aid rapid identification for both of biological weapon attacks and post-attack monitoring during remediation. The final product is envisioned and an automated sample collection and concentration device that will be capable of handling a variety of water matrices ranging from drinking water to wastewater due to the inherent capacity of continuous flow centrifugation to accommodate particle-laden waters and avoid the fouling problem common to all filtration methods when high turbidity water samples are processed. The automated system also will make it possible to perform on-line and real-time monitoring of waterborne pathogens or fecal indicators when the system is coupled with other rapid detection system such as biosensoring techniques.