SBIR/STTR Research Award
FLOURESCENT THIOL PROBES -- CYTOMETRY AND PCR TECHNOLOGY
Award amount
$521,000Program and phase
SBIR · Phase IIAward year
1994Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- Not reported
- Recipient company
- COVALENT ASSOC., INC.
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
WE WILL SYNTHESIZE NEW PROBES FOR THE DETERMINATION OF INTRACELLULAR GLUTATHIONE AND OTHER CELLULAR THIOLS. THE REACTION PRODUCT OF THE PROBES WITH THIOLS WILL HAVE LONGER WAVELENGTH ABSORPTION AND EMISSION AND HIGHER QUANTUM YIELD THAN THE BEST CYTOSOLIC THIOL FLUOROPHORES CURRENTLY IN USE FOR CELL SORTING BY FLOW CYTOMETRY. THE DYES WILL HAVE THE ADVANTAGE OF BEING CELL MEMBRANE PERMEABLE SO THAT CELL LOADING CAN OCCUR AT LOWER EXTRA CELLULAR DYE CONCENTRATIONS THAN CURRENTLY POSSIBLE. THE SYNTHESIZED PROBES WILL BE TESTED IN VITRO AND IN CELL CULTURES FOR ABSORPTION AND EMISSION PROPERTIES WHEN BOUND TO GLUTATHIONE AND OTHER PROTEIN THIOLS. CELL TOXICITY WILL ALSO BE DETERMINED.
Technology keywords
Not reported