SBIR/STTR Research Award
Extracellular Secretion of Proteins in E Coli
Award amount
$72,195Program and phase
SBIR · Phase IAward year
1994Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- Not reported
- Recipient company
- STRATAGENE
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
We will develop a system by which cloned gene products can be efficiently secreted from theE. coli host bacterium into the media. This system would offer significant advantages to conventionalE. coli expression systems since it would permit the isolation of products previously observed to beeither lethal to the host or subject to rapid proteolytic degradation. Furthermore, by remaining withinE. coli, the savings in time, effort, and cost of cloning a gene and expressing its product would beconsiderable.
Technology keywords
Not reported