CHROMOSOME 1P ACCOUNTS FOR APPROXIMATELY 5% OF THE HUMAN GENOME.

CHROMOSOME 1P ACCOUNTS FOR APPROXIMATELY 5% OF THE HUMAN GENOME. THE GENES FOR A NUMBER OF HUMAN DISEASES HAVE BEEN TENTATIVELY ASSIGNED TO THIS REGION. HOWEVER, FIRM ASSIGNMENT IS NOT POSSIBLE FOR MANY OF THESE DISEASES, INCLUDING HEREDITARY MALIGNANT MELANOMA. ACCURATE MAPPING OF THESE GENES REQUIRES THE IDENTIFICATION OF ADDITIONAL MARKERS. THE-STATE-OF-THE-ART APPROACH TO HUMAN GENE MAPPING IS TO IDENTIFY RANDOM SINGLE-COPY DNA PROBES THAT DETECT DNA SEQUENCE POLYMORPHISMS, AND TO ORDER THESE POLYMORPHIC LOCI BY LINKAGE ANALYSIS. DISEASE GENES OF INTEREST ARE THEN MAPPED BY LINKAGE TO THE MARKER LOCI. THE GOAL OF THIS PROJECT IS TO USE RECOMBINANT DNA TECHNOLOGY TO DEVELOP A LIBRARY OF CHROMOSOME 1P RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLPS). IN PHASE I A SMALL NUMBER OF RFLPS LINKED TO THE RH LOCUS WILL BE IDENTIFIED. IN PHASE II THE