Transplacental Detection of Mutagenesis

Exposure of animals and man during development can result in teratogenic, mutagenic, and carcinogenThis research will determine whether transgenic mouse models used for mutagenicity testing can be ustransplacental mutagens. Phase I will be a proof-of-principle study to demonstrate the applicabilityknown mutagen. In Phase II, the assay will be further characterized using additional chemicals and dprotocols, as well as by determining whether or not the mutant spectra of the mutagen of interest istissue of the mother and her offspring. This assay will be used to identify transplacental mutagensopportunity of determining whether mutations which occur during development are related to teratogenoutcomes.