INHIBITORS OF INTERLEUKIN

THIS RESEARCH WILL IDENTIFY AND CLONE ENDOGENOUS INHIBITORY MOLECULES OF INTERLEUKIN-1 (IL-1). HUMAN IMMUNODEFICIENCY VIRUS (HIV)-INFECTED MACROPHAGES AND MACROPHAGE CELL LINES RELEASE TWO INHIBITORS OF IL-1, AN 8-KD MOLECULE AND A 95-KD MOLECULE. POLY (A)(+) MRNA WILL BE PREPARED FROM THESE SOURCES AND CDNA LIBRARIES PREPARED IN LAMBDA GT11 EXPRESSION VECTOR. CLONES WILL BE SCREENED WITH A RABBIT ANTISERUM THAT NEUTRALIZES THE ACTIVITY OF THE 8-KD INHIBITOR. POSITIVE CLONES WILL BE SUBCLONED INTO OKAYAMA-BERG EXPRESSION VECTOR, AND THE ACTIVITY OF TRANSFECTED COS 7 CELL SUPERNATANTS TESTED. IL-1 INHIBITORS HAVE THERAPEUTIC POTENTIAL FOR IMMUNOINFLAMMATORY DISEASES. STUDY OF THESE MOLECULES MAY PROVIDE A BETTER UNDERSTANDING OF THE PATHOGENESIS OF AIDS.