Novel and Efficient Method of Anticancer Drug Delivery

The contents of this proposal are based on the observation that, both in vitro and in vivo, the cytanticancer drugs can be greatly increased if cells are transiently permeabilized, allowing easier paapplication of short and intense pulsed electric fields. The objectives and specific aims of this cuthe data we have already obtained on tumor regression in animal models with murine melanoma cell linbleomycin with electric field. In particular, we will (i) compare different routes of administrationintravenous, and investigate the effect of (ii) different dosages, and (iii) multiple treatments, aloptimized electrical parameters. Results of the combination therapy will be compared with three setsdrug and no electric field, and drug or electric field alone. Apart from usual monitoring and observdetailed histopathological examinations will also be conducted. In addition, field plots with differelectrodes, caliper and needles, will be obtained. The long-term aim of the project is to treat humacancers, particularly advanced melanoma and basal cell carcinoma by this novel method.