SBIR PHASE II TOPIC 294:SITE-SPECIFIC ANTIBODIES FOR GLCNACYLATED PROTEINS IN CANCER; HHSN261201300058C; POP: 9/13/13-09/12/16; CAN: 8478363 IGF::OT::IGF OTHER FUNCTIONS

O GlcNAcylation of proteins plays a critical role in cell cycle regulationapoptosis and signal transductionModification of O GlcNAcylation of proteins by O GlcNAc transferase is reversible by the activity of O GlcNAcaseAddition and removal of O GlcNAc moiety to serine or threonine regulate the function of proteins directly or via decreased phosphorylationO GlcNAcylation of c myc proteins induces ubiquitin dependent c myc degradationGlcNAcylation of pprevents pdegradation and stabilizes pproteinswhich are beneficial in cancer cells by arresting cell growth and inducing apoptosis in response to DNA damageTo elucidate functions of O GlcNAcylation of the proteins it is necessary to develop site specific antibodies for O GlcNAcylated proteinsFew site specific antibodies have been successfully produced so farA lack of site specific O GlcNAcylated Abs prevents facile detection and quantitation of the site to elucidate the function of O GlcNAcylated molecules in physiology and diseaseAvailability of O GlcNAcylation site specific antibodies will facilitate mechanistic studies on O GlcNAcylationand phosphorylation dependent signal transduction pathway in cancerwhich will result in development of O GlcNAcylation inducing anti cancer drugs