IT IS CLEAR FROM A NUMBER OF STUDIES THROUGHOUT THE WORLD THAT HUMAN PAPILLOMAVIRUS (HPV) INFECTION OF THE FEMALE GENITAL TRACT IS A SIGNIFICANT RISK FACTOR IN THE DEVELOPMENT OF CERVICAL CANCER.

IT IS CLEAR FROM A NUMBER OF STUDIES THROUGHOUT THE WORLD THAT HUMAN PAPILLOMAVIRUS (HPV) INFECTION OF THE FEMALE GENITAL TRACT IS A SIGNIFICANT RISK FACTOR IN THE DEVELOPMENT OF CERVICAL CANCER. SINCE HPV CAN BE PRESENT INNORMAL TISSUE SHOWING NO PATHOLOGY, PERHAPS AS A LATENT VIRAL INFECTION, WE PROPOSE TO TEST THE FEASIBILITY OF CONSTRUCTING NUCLEIC ACID PROBES THAT WILL PERMIT THE DEVELOPMENT OF A SIMPLE HYBRIDIZATION TEST FOR THE DETECTIONOF HPVS. IN THIS PHASE I PROPOSAL, SPECIFIC PROBES WILL BE CONSTRUCTED THAT CAN DETECT THE DIFFERENT HPV TYPES BUT THATHAVE NO HOMOLOGY TO EACH OTHER. THE NECESSITY FOR TYPE SPECIFIC PROBES IS DUE TO HOMOLOGY BETWEEN DIFFERENT HPV TYPES WHICH RENDERS FULL LENGTH GENOMIC PROBES UNDESIRABLE FOR TESTING HUMAN SAMPLES. THESE PROBES WILL BE DEVELOPED USING AVAILABLE SEQUENCE DATA AND RESULTS FROM HETERODUPLEX MAPPING THAT INDICATES THE NONHOMOLOGOUS REGIONS BETWEEN DIFFERENT HPV TYPES. AREAS OF INVESTIGATION WILL BE: 1. DEVELOPMENT OF SUBGENOMIC, TYPE-SPECIFIC RNA AND DNA PROBES;2. COMPOSITE PROBES, CONSISTING OF TYPE-SPECIFIC SEQUENCES IN TANDEM; AND 3. SYNTHETIC OLIGONUCLEOTIDE PROBES. PERFORMANCE OF THESE PROBES WILL BE EVALUATED BY SOUTHERN AND DOT BLOT ANALYSIS ON BOTH CLONED VIRAL DNA AND CLINICAL SAMPLES.