SBIR/STTR Research Award
Quantitation of DNA Damage and Repair by Flow Cytometry
Award amount
$50,034Program and phase
SBIR · Phase IAward year
1994Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- Not reported
- Recipient company
- Oncyte, Inc.
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
Our objective is to develop a method for monitoring DNA damage. Damage to DNA in the formof double and single strand breaks will be assessed by fluorescent, in situ nick translation, employingsuspensions of lymphoid cells and a DNA synthesizing system containing E. coli DNA polymerase I.Ionizing radiation will be employed to verify the efficacy of the methodology. We will also determinethe feasibility of this technology to i) study DNA repair, and ii) monitor the radioprotective effects of low,inducing doses of ionizing radiation. Human cell lines with various genotypic configurations of the p53gene will be employed to study the effect of radiation on DNA damage and repair using the nicktranslation technique.
Technology keywords
Not reported