THE GOAL OF THIS PROJECT WILL BE TO SCREEN NEW DERIVATIVES OF L-ASCORBIC ACID ADDUCTS CLASSIFIED AS METHYLFURYLBUTYROLACTONES, ALSO REFERRED TO AS NAFOCARE B.

THE GOAL OF THIS PROJECT WILL BE TO SCREEN NEW DERIVATIVES OF L-ASCORBIC ACID ADDUCTS CLASSIFIED AS METHYLFURYLBUTYROLACTONES, ALSO REFERRED TO AS NAFOCARE B. WORKING IN COLLABORATION WITH A SYNTHETIC ORGANIC CHEMISTRY GROUP AT WEST VIRGINIA UNIVERSITY, WE HAVE DETERMINED THAT THE PARENT COMPOUND, MFBL, HAS EXTENSIVE IMMUNE POTENTIATING ACTIVITY. OUR PROJECT WILL ANALYZE FIVE NEW DERIVATIVES OF L-ASCORBIC ACID IN AN ATTEMPT TO VERIFY WHICH PROSTHETIC GROUPS OTHER THAN THE BUTYROLACTONE STRUCTURE ARE CRITICAL TO BIOLOGICAL ACTIVITY. IN ADDITION, WE HOPE TO DISCOVER NEW SYNTHETIC IMMUNE BIOLOGICAL RESPONSE MODIFIERS. ALL TEST COMPOUNDS WILL BE SCREENED PRESUMPTIVELY USING THE MOUSE LYMPHOCYTE STIMULATION ASSAY. IF THE COMPOUND IS POSITIVE FOR ACTIVITY, FURTHER CONFIRMATORY IMMUNOASSAYS WILL BE PERFORMED USING T-CELL, B-CELL, AND MACROPHAGE EFFECTOR CELLS.