SBIR/STTR Research Award
RAPID DNA EXTRACTION FOR DNA DIAGNOSTICS
Award amount
$49,232Program and phase
SBIR · Phase IAward year
1991Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- Not reported
- Recipient company
- Specialty Laboratories, Inc.
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
THE OVERALL GOAL OF THIS WORK IS TO DEVELOP A RAPID DNA EXTRACTION PROTOCOL THAT CAN BE APPLIED TO HUMAN BODY FLUIDS, SPUTA AND STOOL SAMPLES. THE BASIS OF THE ISOLATION PROCEDURE IS TO SOLUBILIZE THE CLINICAL SPECIMEN WITH THE STRONG DETERGENT SODIUM DODECYLSULFATE (SDS). THE SOLUBILIZATION RELEASES HIGH-MOLECULAR WEIGHT DNA WHICH CAN BE CAPTURED ON MEMBRANE FILTERS. THE IMMOBILIZED DNA IS WASHED FREE OF SDS AND THE DNA ELUTED INTO A POLYMERASE CHAIN REACTION (PCR) BUFFER WHICH CAN IMMEDIATELY BE AMPLIFIED. AFTER OPTIMIZING CONDITIONS FOR SOLUBILIZATION, FILTRATION AND ELUTION, DNA CAN BE RAPIDLY EXTRACTED FROM WHOLE BLOOD THAT IS SUBMITTED TO SPECIALTY LABORATORIES, INC. FOR HIV-1 DNA DETECTION BY THE POLYMERASE CHAIN REACTION.
Technology keywords
Not reported