REGENERATION OF IMMUNOLOGICALLY ACTIVE SURFACES

THE OBJECTIVES OF THIS STUDY ARE TO INVESTIGATE THE BINDING AND ELUTION OF HUMAN, RABBIT, AND MOUSE IMMUNOGLOBULINS (IGG1, F(AB)2 AND FAB) TO SOLID SURFACES AND TO ANTIBODY. THE BINDING AND ELUTION OF ANTIBODY AND ANTIGEN WILL BE STUDIED ON MICROTITER PLATES AND IMMOBILON FILTERS. ELISA ASSAYS FOR HISTAMINE AND DFP WILL BE SET UP TO MEASURE ELUTION OF HAPTEN-ANTIBODY REACTIONS AND TO ASSAY DFP ELUTION FROM ACETYLCHOLINESTERASE. THE THEORETICAL POSSIBILITY OF USING AN ELISA HISTAMINE ASSAY COMBINED WITH SENSITIZED MAST CELLS AS A SYSTEM FOR DETECTION OF CW AND BW AGENTS WILL BE DETERMINED. ALSO THE USE DFP ANTIBODY TO DETECT DFP BOUND TO ACETYLCHOLINESTERASE AND ITS POSSIBLE USE IN DETECTION OF CW AGENTS WILL BE CONSIDERED.