ANIMAL TRIAL OF SEPTIC SHOCK WITH A NOVEL PARS INHIBITOR

Not Available This project addresses needs for identifying and measuring effects of therapeutic agents or toxic agents with sensitive, practical in vitro bioassays employing multi-cellular organisms as Biological Indicators (BI). Our proposal is to develop an innovative bioassay approach based on measuring agents' effects on the metabolic activity in vitro of small waterborne invertebrate BI's, in particular Artemia (brine shrimp), Daphnia, and similar organisms. Novel disposable sensors will be used in our bioassay to measure metabolic activity within BI cultures, serving as me indices of changes in me BI population. Phase I of this project is designed to investigate and establish the feasibility of the proposed bioassay concept in two fundamental aspects: (1) that the sensing approach for a metabolism readout is an efficacious means of assaying multi cellular organisms' activity/health and identifying changes therein induced by exposure to agents; (2) that early stage embryos of Artemia and Daphnia have potential value as a biological-indicator model for assaying agent effects. The goal is to ultimately provide a new methodology presenting opportunities for inexpensive, rapid field bioassays using multicellular organisms potentially prepared conveniently from dry, stable embryos. BENEFITS: This project addresses needs for identifying and measuring effects of therapeutic agents or toxic agents with sensitive, practical in vitro bioassays employing multi-cellular organisms as