SBIR/STTR Research Award
TISSUE SLICES FROM TRANSGENIC MICE-TOXICOLOGICAL TOOL
Award amount
$118,873Program and phase
STTR · Phase IAward year
2001Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- National Institutes of Health
- Recipient company
- VITRON, INC.
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
DESCRIPTION (Adapted from the Investigator's Abstract): The main objective of
this grant proposal is to use precision-cut tissues slices from
genetically-modified mice as an in vitro system for rapid assessment for
potential chemical toxicities. The Phase I goals are to develop two transgenic
mice, one that has an aryl hydrocarbon receptor responsive promoter linked to
green fluorescent protein and another that has an estrogen receptor responsive
promoter linked to yellow fluorescent protein. These mice would facilitate the
development of subsequent in vitro model systems to test the responsiveness of
the constructed promoters to toxic chemicals. After characterization of these
transgenic mice, the PI proposes in Phase II to crossbred these two transgenic
strains containing the aforementioned reporter constructs to generate a
supermouse. Tissues slices from this supermouse can be simultaneously monitored
for a number of toxic events in multiple tissues from a single mouse.
PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE
Technology keywords
Not reported