Lyme Disease Vaccine Using Gonococcal Blebs

The investigators will develop a system in which OspA, an antigen known to induce protective immuneagainst Borrelia burgdorferi, the causative agent of Lyme Disease, is cloned and exported to the outNeisseria gonorrhea, a bacterium that naturally turns over its outer membrane into easily isolated iantigen will be expressed in blebs either as a complete protein or by fusion to another gonococcal cLocalization of the antigen in blebs will be characterized and the blebs used in animal studies to dimmunogenicity of the construct as well as its potential ability to elicit protective immune respons