Genetic Selection of Peptide Cleaving Antibodies

We will apply our genetic selection procedure to facilitate rapid production of catalytic antibodiecleaving any given peptide. The technology involves cotransformation of E. coli with a specially desrendered infection incompetent together with a combinatorial library cloned into a phagemid. Only phcatalytic antibodies of the desired specificity are packaged into infectious phage particles and thutechnology can be expected to obviate the need for design and synthesis of transition state analogsconstruction of separate libraries for each target peptide. Ultimately, these antibodies will serveviral diseases. Catalytic antibodies selected by this procedure should provide a significant advantamonoclonal antibodies since each antibody can neutralize multiple targets, thus reducing the requirenoted that this technology can be applied to the selection of human antibodies as easily as murine.