SBIR/STTR Research Award
Mucosal Immunity Against Non-infectious SIV Particles
Award amount
$49,999Program and phase
SBIR · Phase IAward year
1993Award details
- Awarding agency
- Department of Health and Human Services
- Branch
- Not reported
- Recipient company
- Secretech, Inc.
- Proposal award date
- Not reported
- Contract end date
- Not reported
- UEI
- Not reported
- Solicitation number
- Not reported
- Topic code
- Not reported
Research abstract
The long term objective is to induce a protective mucosal immune response to prevent HIV-1 infection. We will immunize orally with recombinant defective SIV-like particles entrapped in microcapsules of lactige-glyco- lide copolymer in order to stimulate a mucosal immune response in mice. The effectiveness of the microcapsule in protecting the immunogenicity of the gag-env will be established by treating the microcapsules with stimulated gastric and intestinal juices, and the resulting protein will be characterized by immunoblot and SDS-PAGE analysis. The optimized microcapsules will be administered to mice orally in parallel with gag-env in solution. The antibody response in serum, saliva and lavages from the gut and the vagina of immunized mice will be determined. In Phase II, we will study the response of immunized macaques challenged with SIV through the vaginal route.
Technology keywords
Not reported