Concentration Measurements of Biomolecules

Laser Induced Fluorescence (LIF) can be used as a diagnostic device in biology and medicine. In gendifficulties relating the fluorescence spectra to the concentration of the emitters within the tissuspecies, and in the possibility of destroying the molecules being probed if the wrong excitation wavthe problems associated with fluorescence can be eliminated by the use of Raman scattering. Raman temeasurement of multiple species even in aqueous solutions (not accessible by infra red techniques beabsorption). However, spontaneous Raman scattering techniques generate very weak signals. Signals cathrough the use of resonance Raman techniques. Both of these methods still suffer from the fact thatextent temporal information is lost. Spatial information can be recovered through the use of confocaresearch is intended to investigate a non-invasive, real-time, high resolution device for the measurlevels of enzymes, metabolites or other critical molecules. This unique device is based on nonlinearsimultaneously measure the concentrations and temporal evolution of several molecular species in indcells.