Novel Protein Kinase Substrates

Protein kinases play a key role in the regulation of metabolic activities in eukaryotic cells. A laprotein kinases have been isolated and characterized, but we are still in the early stages of undersphosphorylation on the target proteins. Better tools are needed to study protein kinases and the prowill design and manufacture a new generation of protein kinase substrates that will facilitate the aenzymes. These new substrates will enable the simultaneous assay of multiple protein kinases and wilnewly-discovered ones. The substrates consist of artificial proteins with a constant N-terminus taggpurification. A variable central region that is a polymer of a specific decapeptide and a specific Coptimal substrate for a protein kinase. The only phosphorylatable site will be in this C-terminal reC-terminal region to a specific length of the central region, many different kinase substrates can bdisplayed simultaneously by SDS-PAGE. These artificial proteins may also be valuable as markers in tweight range.