Mabs Specific to Serotonin 5-HT2 Receptors

Serotonin (5-hydroxytryptamine; 5-HT) mediates many central and nervous system functions by its intwith specific neuronal receptors. It is well established that 5-HT receptors are related to anxiety5-HT receptors are also binding sites for hallucinogenic drugs. Physiological functions such as blooand platelet shapes are also regulated by 5-HT receptors. Physiological, pharmacological, and molecuhave identified thirteen serotonin receptors belonging to seven subtypes. The molecular cloning of sprovides a great understanding of the structure and function of these pharmacologically important rereceptors may be considered as a protoype for the study of serotonin receptors. A better understandilocalization and regulation of 5-HT receptors may lead to development of receptor specific drugs. MAavailable to different 5-HT receptor subtypes. Using synthetic peptides and recombinant proteins asmonoclonal antibodies will be made to 5-HT2 receptor subtypes. Antibodies will be screened for theirimmunohistochemistry, western blot, and immunoprecipitation. Monoclonal antibodies to the 5-HT2 receas a valuable adjunct to classical radioligand binding for immunolocalization and regulation studies