Triple Helix-Based Acoustic Plate Mode DNA Biosensor

BIODE will utilize the results of its Phase I research to develop (Phase II) and commercialize (Phase III) a totally new solid state biosensor technology for biological measurements. This technology is based upon the merging of two technologies: Triple Helix Nucleic Acid Probes with Acoustic Plate Mode (APM) micro sensors. Recent advances by BIODE in the design and operation of APM devices have resulted in a DNA hybridization sensor principle with excellent sensitivity (nanograms/milliliter), selectivity and temperature stability when used with a model probe system. BIODE has received further funding from the DOE to optimize the electrical properties of the device. We will also attempt the direct electronic in situ sensing of a diagnostically significant DNA gene sequence, which codes for the abundant late matrix antigen of Cytomegalovirus. A known short-coming of the previous work is the need to denature naturally-occurring dsDNA to single strands before the sensor is able to detect it. The current project addresses the issue of dsDNA detection via triple helix capture. The currently-funded DOE contract will provide BIODE with an extremely sensitive detection element, and the work will provide an improved technique for imparting selectivity to the device.