IMMUNODIAGNOSTIC FOR MEASURING HUMAN CELL PROLIFERATION

THE EVALUATION OF TISSUES FOR THE PRESENCE OF CANCER CELLS IN ABNORMAL TISSUES IS AN IMPORTANT AND CRUCIAL STEP IN THE DIAGNOSIS, DISEASE MANAGEMENT, AND CONTROL OF CANCER. CBR LABORATORIES, INC., HAS GENERATED A MOUSE MONOCLONAL ANTIBODY THAT DETECTS A GLYCOLIPID ANTIGEN (PAA) ON PROLIFERATING T CELLS, B CELLS, AND TUMOR CELLS IN BOTH FROZEN AND PARAFFIN-FIXED TISSUES. IT IS HYPOTHESIZED THAT THE LEVEL OF BINDING OF THIS ANTIBODY TO A CELL REFLECTS ITSPROLIFERATION POTENTIAL. THIS CONCEPT WILL BE TESTED IN A SERIES OF EXPERIMENTS THAT INCLUDE IN SITU COMPARATIVE IMMUNOHISTOCHEMICAL STAINING OF PROLIFERATING CELLS IN LYMPHOMAS AND A VARIETY OF SOLID TUMORS WITH ANTI-PAA MONOCLONAL ANTIBODY THAT IS USED TO STAIN PROLIFERATING CELLS IN FROZEN SECTIONS OF NORMAL AND CANCER TISSUE. DIGITAL IMAGE ANALYSIS AND IMMUNOFLUORESCENCE STAINING WILL BE USED FOR THE IN SITU STUDIES. THE RESULTS OF THESE STUDIES WILL DETERMINE THE FEASIBILITY OF USING ANTI-PAA MONOCLONAL ANTIBODY AS AN IMMUNODIAGNOSTIC REAGENT FOR THE DETECTION OF PROLIFERATING CELLS IN TUMORS.